白屈菜提取物中生物碱的镇痛抗炎作用研究

Analgesic and Anti-inflammatory Effect of the Alkaloid in Chelidonium majus Extract

目的:研究白屈菜提取物的镇痛抗炎作用。方法:小鼠分别ig给予白屈菜提取物低、中、高剂量(0.1,0.2,0.4g·kg-1)1次,采用扭体法、热板法、福尔马林致痛模型,观察白屈菜提取物的镇痛作用。按以上剂量连续7 d,采用小鼠二甲苯耳肿胀模型和小鼠腹腔毛细血管通透性实验,观察其抗炎作用;通过纳洛酮拮抗实验,初步验证其镇痛机制。结果:白屈菜提取物可明显抑制冰醋酸引起的小鼠扭体反应次数和扭体反应鼠数(P<0.05),提高小鼠光热辐射致疼痛反应的痛阈值;高剂量组对二甲苯引起的小鼠耳廓肿胀有显著的抑制作用(P<0.01)。结论:白屈菜提取物具有显著镇痛抗炎作用,其镇痛作用不受纳洛酮拮抗,主要是外周性的。

Objective：To study the anti-inflammatory,analgesic effects of Chelidonium majus extract.Method：Writhing method,hot-plate method and formalin pain model were used to evaluate the analgesic effect.The inhibition of auricle edema induced by xylene and mouse peritoneal capillary permeability experiment were used to assess the anti-inflammatory effect.Naloxone test was used to interpret the analgesic mechanisms of the extract.Result：High dose group of C.majus extract significantly decreased the twisting times in mice induced by acetic acid（P0.05）,and obviously inhibited the swelling of mouse pinna induced by xylene（P0.01）.Conclusion：C.majus extract has significant analgesic and anti-inflammatory effects.Its analgesic effects are mainly peripheral and not antagonized by Naloxone.