ERIC-PCR与PFGE检测铜绿假单胞菌同源性的方法学比较

The methodology comparison of ERIC-PCR and PFGE used in Pseudomonas aeruginosa homology detection

目的比较肠杆菌基因间重复一致序列PCR(ERIC-PCR)与限制性内切酶联合脉冲场凝胶电泳(PFGE)检测铜绿假单胞菌同源性的方法学一致性。方法分别用ERIC-PCR和PFGE对北京某医院48株铜绿假单胞菌株进行分型,明确其是否有克隆传播。结果 ERIC-PCR方法鉴定有34株为同一克隆来源,其余14株被分为9种基因型。PFGE方法分型鉴定有38株为同一克隆来源,其余10株表现为7种基因型,均提示脑外科内的铜绿假单胞菌在2010年存在一个克隆株传播。两种方法学结果的符合率为89.5%,PFGE重复3次结果相似度达96.6%,ERIC-PCR重复5次结果相似度达75.4%。结论 ERIC-PC操作简便快速,重复性较好,与PFGE结果一致性高,适合在基层医院进行细菌流行病学分析。

Objectives Compare the methodology consistency of PFGE and ERIC-PCR used in Pseudomonas aeruginosa homology detection. Methods Pulse-field gel electrophoresis （PFGE） and Enterobacterial repetitive intergenic consensus polymerase chain reaction （ERIC-PCR） were performed respectively to detect molecular features of 48 pan-drug resistant Pseudomonas aeruginosa strains. Results In all, 34 strains were proved to be the same clone by ERIC-PCR, and the other strains were identified into 9 types; 38 strains were presented the same clone by PFGE, and the rest were classified into 7 types. The results showed that the same cloning strain was emerged in department of cerebral surgery in 2010. The coincidence rate of the two methods was 89.5%. PFGE had three repetitions, and the similarity was 96.6%; ERIC-PCR had five repetitions, and the similarity was 75.4%. Conclusion ERIC-PCR, with rapid detection, good reproducibility and high consistency with PFGE, is easy and simple to handle. It is suitable for bacterial epidemiological analysis in primary hospital.