限制性液体复苏对出血未控制性休克大鼠血浆内皮素和一氧化氮的影响

The effects of limited fluid resuscitation on nitric oxide and endothelin in rats with uncontrolled hemorrhagic shock

目的探讨限制性液体复苏对出血未控制性休克(UHS)大鼠内皮素(ET)和一氧化氮(NO)的影响。方法将36只SD大鼠均建立UHS模型,随机分成对照组(A组)、限制性复苏组(B组)、积极复苏组(C组)。模拟创伤救治过程,A组大鼠未行液体复苏,B、C组进行不同方法液体复苏,2 h后检测门静脉血ET、NO浓度,出血量、小肠黏膜组织病理学评分。结果 A组血ET、NO浓度、出血量、小肠病理组织学评分均高于B、C组,B组各指标明显低于C组。结论限制性液体复苏较积极复苏更能减轻实验性UHS大鼠门静脉血ET、NO浓度及E/N比值,减少肠黏膜损伤,可能对改善UHS大鼠微循环障碍更有益。

【Objective】 To explore the effects of limited fluid resuscitation on rat with uncontrolled hemorrhagic shock（UHS）.【Methods】 36 SD rat were made the model of UHS and they were divided randomly into 3 groups which used different therapies： control group of rats with UHS were not given fluid resuscitation（A）,the group of rats with UHS received limited fluid resuscitation（B）,the group of rats with UHS received aggressive fluid resuscitation（C）.After 2 h,all of rats were measured the concentration of ET and NO in portal vein blood,amount of bleeding,and given histopathology scoring of intestinal mucosal.【Results】 There was a difference among 3 groups in the concentration of ET and NO,amount of bleeding,histopathology scoring,group A was higher significantly than group B and C,and group B was lighter than group C.【Conclusion】 Compared with aggressive fluid resuscitation,the limited fluid resuscitation can reduce significantly the concentration of ET,NO in portal vein blood and NO/ET levels for rats with UHS,and decrease the damage of intestinal mucosa and benefit for improvement of microcirculation disturbance.