杂草稻中胚轴伸长的细胞形态学观察

Observation of Mesocotyl Cell Morphology of Weed Rice

利用人工气候箱,在黑暗条件下培养幼苗,采用石蜡切片法对杂草稻WR04-6和栽培稻秋光的中胚轴伸长进行细胞形态学观察。研究结果表明:杂草稻幼苗的中胚轴只有在黑暗条件下才进行伸长生长,两材料幼苗初期苗高的差异主要来自中胚轴的伸长。中胚轴伸长初始阶段,其细胞数目达到整个时期数目的 80%以上,随后分裂增殖基本停止;杂草稻WR04-6在纵向长度上细胞数目比秋光高100%,而细胞长度比秋光长400%以上;初期细胞分裂增殖和细胞伸长共同促进中胚轴伸长生长,后期则依靠细胞纵向延伸促进中胚轴伸长生长。杂草稻中胚轴的伸长生长由细胞数量增加和细胞伸长共同起作用,细胞长度纵向延伸对中胚轴伸长生长起决定作用。

For observing the cytomorphology of mesocotyl on the dark condition in artificial climate chamber, we used rice varieties WR04-6 and Akihikari. The elongation growth of seedling mesocotyl could occur only at dark condition. The differences of height between varieties at seedling stage were caused by mesocotyl elongation, The mesocotyl cell morphology of WR04-6 and Akihikari observed with the paraffin method showed that the number of mesocotyl cell reached 80% of total and then cell division and proliferation ceased. The cell number of longitudinal and cell length of long mesocotyl material were 100% and 400% as those of short mesocotyl material respectively. The cell division and elongation promoted elongation growth of seedling mesocotyl together during early stage of that and then it depended cell longitudinal extending later. The added cell number and cell elongation promoted mesocotyl elongation together while cell longitudinal extending made a key effect on elongation growth of mesocotyl.