红色叶王族海棠成色色素分析

Pigments Analysis of Malus 'Royalty' Red Leaves

利用紫外可见分光光度计、薄层层析色谱法、高效液相色谱仪-二极管阵列检测器(HPLC-DAD)对王族海棠叶片色素进行了鉴定,并对鉴定出的单体酚、叶绿素、类胡萝卜素以及总黄酮含量进行定量分析。结果表明:王族海棠叶片内含有叶绿素、类胡萝卜素、矢车菊素半乳糖苷、矢车菊素及其它单体酚(儿茶素、绿原酸、表儿茶素、芦丁、对香豆酸、二氢槲皮素、木犀草素、槲皮素、山奈酚、根皮素、柚皮素)。其中,矢车菊素半乳糖苷和矢车菊素为王族海棠叶片呈现红色的主要色素,叶绿素起到使叶色加深的作用,类胡萝卜素、芦丁、木犀草素、槲皮素、山奈酚、柚皮素等起着辅助成色的作用。

Pigments of Malus ‘Royalty＇ leaves were qualitative identified using of UV photo-spectrometer,thin layer chromatography and high performance liquid chromatography;and quantitative analysized using of NaNO2-Al（NO3）3-NaOH coloration system and HPLC-DAD.The results showed that Malus ‘Royalty＇ leaves contains chlorophyll,carotenoids,cyanidin,cyanidin-galactoside and other monomer phenol（catechin,chlorogenic acid,epicatechin,rutin,ρ-coumaric acid,dihydroquercetin,lueolin,quecetin,kaempferol,phloretin,naringenin）.The red color of Malus ＇Royalty＇ leaves was caused by accumulation of cyanidin,cyanidin-galactoside and chlorophylls.Carotenoids,rutin,lueolin,quecetin,kaempferol,naringenin played a role as co-pigments.