黄瓜亲本与其杂交种F_1代的同工酶比较分析

Comparing Study on the Isozyme of Hybrid F_1 and Parents of Cucumis sativus L.

选用2个强优势华北型黄瓜杂交种F1(9514、9518)及其亲本,对子叶期和苗期过氧化物酶和酯酶的同工酶进行了电泳分析。结果表明:利用黄瓜子叶期和幼苗期过氧化物酶和酯酶的同工酶电泳酶谱对黄瓜的杂种优势进行早期预测,不是很有效的途径;通过电泳酶谱分析表明,黄瓜的遗传差异较小,遗传基础相对狭窄,遗传变异率低。在杂交育种中,应尽可能选配遗传差异大的亲本,以扩大黄瓜杂交种的遗传基础,增强杂种后代对环境的适应性、抗逆性。

Two crosses F1 in North China（9514,9518） with strongly heterosis and their parents were studied on the isozyme electrophoresis pattern of peroxidase and esterase in the experiment.The results showed that it was not effective measurement to predict the cucumber heterosis with the isozyme electrophoresis pattern esterase and peroxidase in the cotyledon and seedling stage.It was relatively narrow that the genetic basis of the cucumber after the analysis of the electrophoresis pattern.In the breeding process,it should be possible to select genetic differences in parents of hybrid cucumber,to expand the genetic basis of hybrid progeny,enhance the adaptability to the environment.