分光光度法测定高浓度培养液下的产油酵母菌生长曲线

Growth Curves of Oleaginous Yeast in High Density Liquid Medium Determined by Spectrophotometry

采用分光光度法测定酵母菌不同生长时间高浓度培养液的OD600值,并对不同生长时间高浓度培养液进行相应倍数的稀释,使用建立OD600值回归方程的方法,利用回归后的OD600值绘制出产油酵母菌Y1的生长曲线。结果表明:所得到的生长曲线可以准确反应菌体的生长规律。酵母菌Y1在适宜生长条件下菌体细胞生长出现4个阶段:延滞期、对数期、稳定期和衰亡期,其中,0～4h为延滞期,4～24h为对数生长期,24～68h为稳定期,68h以后进入衰亡期。该研究旨在为相关研究人员提供类似问题的详细解决办法和重要参考。

The oleaginous yeast OD600 was determined through the spectrophotometer in different growth time which was in the high density liquid medium,and the high density liquid medium was diluted in different dilution which was in different growth time.Using the method of establishing set of regression equation of OD600,yeast Y1 growth curve was drawn using the OD600 values after the reunification.The results showed that the growth curve can accurately reflect the cell growth regular.Bacterial cells of yeast Y1 occurred four stages under suitable growth conditions,lag phase,0~4 hours;logarithmic phase,4~24 hours;stationary phase：24~68 hours;decline phase：after 68 hours.This research aimed to provide research solutions to similar problems in detail and important information for related researchers.