猪眼基底部玻璃体视网膜界面的超微结构观察

Ultrastructure of basal vitreo-retina interface in enucleated pig eyes

目的:观察离体猪眼玻璃体腔内注射酶后基底部玻璃体视网膜界面的超微结构。方法:取新鲜尸体猪眼130眼,随机均分A,B,C,D及对照组共5组,每组再分为2小组,每小组各13只。其中A和B组为透明质酸酶(hyaluronidase,HA)组,分别于玻璃体腔内注射终浓度为200,800U/mL的HA0.1mL;C和D组为软骨素酶(chondroitinase,CA)组,分别于玻璃体腔内注射终浓度为10,50U/mL的CA0.1mL;对照组玻璃体内注射磷酸缓冲盐(PBS)0.1mL。各组的眼球分别在37℃下水浴15min和30min后取出,经4%戊二醛和自配视网膜固定液固定,行石蜡切片苏木素-伊红染色检查、基底部扫描电镜检查及透射电镜检查,观察基底部玻璃体视网膜情况。结果:病理检查显示,大体标本和切片均见基底部玻璃体有部分液化、降解;扫描电镜显示,CA50U/mL组和HA800U/mL组均可见基底部玻璃体与对照组相比有显著减少;透射电镜显示,实验组(B,C,D组)各组玻璃体视网膜界面残余纤维较对照组明显减少。结论:体外使用HA和CA均可诱导猪眼基底部玻璃体脱离,但CA可能引起的眼内副作用更大。

AIM： To investigate the ultrastructure of the basal vitreo- retina interface in enucleated pig eyes after vitreous injection of enzymes. METHODS：Totally 130 eyes were obtained from freshly slaughter pigs and divided into 5 groups. Each group was sub-divided into the 15 minutes group and the 30 minutes group, with 13 eyes in each group. The control group were performed middle vitreous injection of 0. lmL PBS, 200U/mL and 800U/mL hyaluronidase（HA） were injected in groups A and B, 10U/mL and 50U/mL chondroitinase （CA） in groups C and D. After incubation for 15 minutes and 30 minutes, the eye balls were fixed with 4% glutaraldehyde and retina fixative solution. Pathological examination, eosin-hematoxylin staining, scan electron microscopy were taken to evaluate the remaining vitreous on vitreous base, transmission electron microscopy to evaluate retina toxicity. ~ RESULTS： Both of gross appearance and hematoxylin＋ eosine slice all revealed basal vitreous partially liquefied and degradation. Remarkably decrease was found in basal vitreous than the control group both in the HA 800U/mL group and the CA 50U/mL group when using electron microscopy scanning. Transmission electron microscopy in group B, C and D revealed the remaining vitreous were less than the control group. CONCLUSION： Both CA and HA can cause basal vitreoretinal detachment in enucleated pig eyes. But the HA may cause less damage to the retina.