摘要
该实验从中缅树鼩(Tupaia belangeri)组织中成功克隆Klf4、Sox2和c-Myc基因的部分序列,长度分别为382、612和485bp,分别编码127、204和161个氨基酸。树鼩的Klf4、Sox2和c-Myc序列与人相应序列的相似性分别为89%、98%和89%。将树鼩Klf4、Sox2和c-Myc基因分别与其他物种的这3个基因进行系统进化分析,发现Klf4和Sox2基因所构建的系统进化树结构较为一致,但与c-Myc所构建的系统进化树有所不同,表明这些基因在进化上存在差异。Klf4、Sox2和c-Myc基因的克隆为进一步研究其功能奠定了基础。
In this paper, partial sequences of the tree shrew （Tupaia belangeri） Klf4, Sox2, and c-Myc genes were cloned and sequenced, which were 382, 612, and 485 bp in length and encoded 127, 204, and 161 amino acids, respectively. Whereas, their eDNA sequence identities with those of human were 89%, 98%, and 89%, respectively. Their phylogenetic tree results indicated different topologies and suggested individual evolutional pathways. These results can facilitate further functional studies.
基金
科技部重大科学研究计划重大科学问题导向项目(2012CBA01300
杨世华)
教育部"新世纪国家优秀人才"支持计划项目
国家自然科学基金项目(31071279
30871232)
云南省科技创新人才计划项目(2011CI009)
