北京地区成人呼吸道感染患者中HCoV-HKU1感染的初步分析与基因型确定

Characterization of the Infection and Genotype Distribution of Hu man Coronavirus HKU1 Among Adults with Acute Respiratory Tract Infection in Beijing

目的：对北京地区成人呼吸道感染患者中人冠状病毒（HCoV）HKUl的感染状况进行初步分析，了解其流行分布、临床特点，并确定流行株的基因型别。方法：2011年1月～2012年1月从北京地区成人呼吸道感染患者中收集鼻咽拭子标本559份，利用靶向棘突蛋白S与核心蛋白N的2种巢式PCR方法进行HCoV—HKUl筛查，并对阳性片段进行序列测定，以确定其基因型别；同时，对阳性标本进行常见呼吸道病毒的共感染筛查，进而分析HCoV—HKUl感染的临床表现和流行病学特点。结果：559份鼻咽拭子标本中检出HCoV—HKUl阳性9例（1．61％），其中3例合并HCoV-0C43感染；感染患者临床表现为急性呼吸道症状，以发热（88．9％）、咽痛（66．7％）、寒颤（68％）、流涕（55．6％）和咳嗽（44_4％）为主，其中1例有系统性红斑狼疮史；阳性基因片段系统进化分析发现这9例感染均为HCoV—I-IKUlB型。结论：北京地区近年成人呼吸道感染患者中HCoV—HKUl感染率较低（1．61％）。其流行株基因型为B型。

Objective： To study the molecular epidemiological and clinical profiles of human coronavirus（HCoV） HKU1 among adults with acute respiratory infection（ARI） in Beijing. Methods： The nest RT-PCR assay targeting to spike（S） and nucleocapsid（N）coding region of HCoV-HKU1 was developed and used for screening HCoV- HKU1 infection among 559 nasopharyngeal swab specimens, which collected from adults with ARI in Beijing be- tween January 2011 and January 2012. The demographic and clinical data associated with HCoV-HKU1 infection were examined. Co-infection with other HCoV or respiratory viruses was also investigated by RT-PCR or multiple RT-PCR. The genotype distribution of HCoV-HKU1 infection was identified by sequence analysis of PCR positive products. Results： 9 of 559（1.61%） specimens were detected as positive for HCoV-HKU1 by nest RT-PCR, 3 of them were found co-infected with HCoV-OC43. The clinical profiles were indicated as fever（88.9%）, pharyngitis （66.7%）, rigors（68%）, nasal discharge（55.6%） and cough（44.4%）, and one of 9 patients with SLE. Sequence analysis showed that all of the HCoV-HKU1 infections fell into genotype B. Conclusion： Our data showed that HCoV-HKU1 prevalence was low（1.61%） among adults with ARI in Beijing area, the genotype distribution of HCoV-HKU1 was identified as B.