实时荧光核酸恒温扩增技术和液体培养法检测解脲脲原体的比较

Comparison of Simultaneous Amplification and Testing and Liquid Culture in the Detection of Ureaplasma Urealyticum

目的:对实时荧光核酸恒温扩增技术(SAT)和液体培养法检测解脲脲原体(UU)的结果进行评估,以选择更为快速、准确、实用的临床检测方法。方法:共采集180例疑似泌尿生殖道感染患者的尿液及2份拭子样本,一份拭子样本用于液体培养,另一份拭子和尿液样本用于SAT检测。结果:液体培养和尿液SAT检测阳性率均为61.1%,拭子SAT检测阳性率为63.3%,其中16例拭子培养和拭子SAT检测结果不一致,18例拭子培养和尿液SAT结果不一致,但与拭子培养比较,拭子和尿液SAT结果均无统计学意义(P>0.05,kappa>0.75)。结论:SAT检测UU可以尿液为样本,检测效能与液体培养和拭子SAT基本相当,但尿液SAT法取样方便,检测快速,适于临床实验室对UU的检测。

Objective： To compare simultaneous amplification and testing（SAT） with liquid culture in the detec- tion of Ureaplasma urealyticum（UU）, thus to select a more rapid, accurate, and practical method for diagnosis of UU. Methods： Swab and urine samples were collected from 180 patients with suspected urogenital tract infection. The swab was tested by liquid culture and SAT, while urine by SAT. Results： The swab positive rate detected by liquid culture and urine positive rate by SAT were the same（61.1%）, while swab positive rate detected by SAT was 63.3%, which there was no significant difference（P〉0.05, kappa〉0.75）. The results of 16 patients were incon- sistent between swab detected by liquid culture and SAT. The results of 18 patients were inconsistent between swab detected by liquid culture and urine by SAT. Conclusion： SAT is a convenient, rapid and accurate method for detection of UU in urine from patients with urogenital tract infection, which is suitable for clinic laboratory di- agnosis.