西双版纳糯玉米地方品种遗传多样性分析

Genetic Diversity of Waxy Maize Landraces in Xishuangbanna

利用SSR标记对西双版纳的36个小糯玉米地方品种和2个对照品种进行遗传多样性研究。从800余对SSR引物中筛选出100对多态性好、稳定性高的SSR引物。结果表明,这100对SSR引物在36个糯玉米地方品种中共检测出353个等位基因,每对引物检测出2～8个,平均为3.53个,平均多态性息量(PIC)为0.53,平均标记索引系数(MI)为2.00。聚类分析表明,糯玉米地方品种聚为6个类群,与对照品种相比,地方品种独立成群。

The genetic diversity of waxy maize landraces in Xishuangbanna were analyzed by SSR markers. Thirty-six waxy maize landraces and two control cultivars were applied in the study, a hundred SSR primers were screened from 800 SSRs, which could produce stable and polymorphic bands. The results showed that 353 alleles were detected in 36 waxy maize landraces. The average number of alleles per SSR primer was 3.53, ranging from 2 to 8. The average polymorphism information content was 0.53, average marker indices 2.00. Cluster analysis showed that the 36 waxy maize landraces divided into 6 groups, and compared with CK, the local varieties of independent communities. This study provided a basis for the protection the waxy maize landraces genetic resources in Xishuangbanna and waxy maize cultivar selection.