摘要
通过对纳豆激酶分离纯化方法的研究,得出:纳豆激酶发酵液经离心、30%～70%硫酸铵分段盐析沉淀、Sephadex G-50凝胶层析和QAE-Sephadex A-50离子交换层析后,得到的酶液经SDS-PAGE电泳检验为单一条带,分子量在27～28ku之间。本纯化方法的回收率达25.07%,纯化倍数为13.09。
In order to obtain high purity nattokinase,some separation and purification techniques were explored.The optimized process includes：removing cells by centrifugation,30%～70% saturation ammonium sulfate precipitation,gel filtration chromatography with Sephadex G-50 and ion-exchange chromatography with QAE-Sephadex A-50.The purified enzyme showed a single protein band in the SDS-PAGE and its molecular weight was 27～28 ku.The purification factor and activity recovery of the nattokinase are 13.09 times and 25.07%,respectively.
出处
《食品科技》
CAS
北大核心
2013年第4期241-244,共4页
Food Science and Technology
关键词
纳豆激酶
分离纯化
玉米烯酮
凝胶层析
离子交换层析
nattokinase
separation and purification
zearalenone
gel chromatography
ion-exchange chromatography
