应用酶热生物分析仪快速测量血糖的研究分析

The investigation analysis blood glucose fast determination with enzyme thermistor bioanalysis equipment

目的通过酶热生物分析仪与检验科全自动生化分析仪血糖检测结果的对比,验证此仪器的精准度。长远目的在于根据热传感器的特性开发一种半连续的检测葡萄糖浓度的床旁监测系统。方法以实验室大型生化分析仪作对比,应用单通道酶热生物分析仪对海军总医院神经外科150例患者进行全血的快速血糖测定。结果两种检测方法在检测血糖中差异无显著性(P>0.05),应用单通道酶热生物分析仪快速测定血糖方法可靠,此检测方法与临床检测结果基本保持一致。每次测量时间约3 min。可以连续测量45 d而酶的活性不变。结论酶热生物分析仪可以用来监测血糖指标及评估治疗效能,并且有能力在不需要任何预处理的全血中实施快速的动态检测。

Objective To compare the enzyme thermistor bioanalysis equipment with blood glucose meter which is analyzed by automatic biochemical analyzer, validate the correct rate of this equipment. The long - term aim of the project was to develop a prototype for a bedside moni- tor system for semi - continuous monitoring of the blood glucose concentration. This was made possible by using the special advantage of the ther- mal sensor technique in combination with the adjustment of flow. Methods 150 patients were collected in our department. The glucose level was determined using a single channel enzyme thermistor device. Then the results were compared with lab biochemistry analysator. Results There was no significant difference between these two methods （ P 〉 0.05 ）. The measuring method with single channel enzyme thermistor device is a reliable method in determining blood glucose level. Single channel enzyme thermistor keep a coincidence with automatic biochemical analyzer. The assay cycle time was 3 minutes. Comparative analysis between our device and the clinical system device showed an excellent correlation for 150 patient blood samples. The device was stabile for hundreds of injections over a period of 45 days. Conclusion Determined glucose in whole blood using a single channel Enzyme Thermistor device can be used to assess the severity of an illness and for evaluating therapeutic efficacy. The ability of the device to analyze whole blood without any pretreatment makes it possible to develop real -time analysis.