补阳还五汤对易损斑块模型家兔心脏和肝脏组织学的影响

Effect of Buyang Huanwu decoction on histology of heart and liver in rabbits with vulnerable plaque

[目的]探讨补阳还五汤对动脉粥样硬化易损斑块模型家兔心脏和肝脏组织学的影响。[方法]通过喂养高脂饲料和主动脉球囊内皮拉伤术复制动脉粥样硬化易损斑块模型,实验分为假手术组,模型组,补阳还五汤组,历时10周。称质量,处死后,称取心肝质量,计算心体指数和肝体指数。取部分心肝组织行苏木精-伊红(HE)染色,观察镜下病变。[结果]1)心体指数和肝体指数的观察:补阳还五汤能够有效降低心体指数和肝体指数,与模型组相比差异有统计学意义(P<0.05)。2)HE观察:补阳还五汤可以有效减轻心肌细胞和肝细胞水肿。[结论]补阳还五汤可以减轻易损斑块模型家兔心肝组织的细胞水肿,其机制尚待进一步研究。

[Objective] To observe the effects of Buyang Huanwu decoction on the histology of hem1 and liver in rabbits with vulnerable plaque of arterial sclerosis. [Methods] The vulnerable arterial sclerutic plaque was reproduced by feeding diet with high cholesterol lipid and injuring the endolhelium of aortic saeeulus by pulling. The rabbits were divided into three groups： sham operation group, model group and Buyang Huanwu deeuction gruup. The experiment was lasted for 10 weeks. At the end after weighing the, animals were killed. Weighling lhe hear＇d and liver and ealeulating the cardiac somatic index （C1）and hepatosomatie index （HI）we, re perfnrmed. The samples of heart and liver were collected tk,r Hematoxylin-eosin （HE） staining and the histulogical observation. [Results] 1 ）Buyang Huanwu decocliou could effectively reduce both CI and HI compared with the model group （P〈0.05）. 2）The histological observation found thai Buyang Huanwu decoction could effectively reduce the edema of myoeardial cell and liver cell. [Conclusion] Buyang Huanwu decoction can efficlively reduce the swelling of the myocardial cells aud hepatic cells, and the mechanism of which remains further studied.