响应面法优化零陵香水溶性多糖组分提取工艺及其抗炎作用的研究

Extract technique of water-soluble polysaccharide of Lysimachia foenum-graecum Hance optimized by RSM and its anti-inflammatory actions

[目的]优化零陵香水溶性组分中多糖的最佳提取工艺，研究其抗炎效果。[方法1应用DesignExpert软件响应面法（RSM）对零陵香多糖的提取工艺进行优化，以最佳提取工艺提取零陵香多糖，并进行抗炎实验。[结果]零陵香多糖浸提的最佳工艺条件为：温度98℃，时间20min，液料比30mL／g，实际最大提取率为1．28％，与预期的1．34％拟合度较高。零陵香多糖在0．75～3．00g／kg（生药）浓度范围具有抗炎能力。[结论]响应面法可应用于零陵香多糖提取的工艺优化；其抗炎效果与剂量成呈正相关I生。

[Objective] To optimize the excellent extracted technique of water-soluble polysaccharide of Lysimachiafoenum-graecum Hance （LP） by RSM and study its anti-inflammatory actions. [Methods] Using Design-Expert （RSM） the extract technique were optimized and the PL was extracted with the obtained technique. The anti-inflammatory actions of LP were observed. [Results] The best factors expected （yield 1.34%） were 98 ℃, 20 min, 30 mL/g, and the real yield was 1.28%. LP had anti-inflammatory actions within the range of 0.75-3.00 g/kg. [Conclusion] RSM can be used for LP extraction and the actions of anti-inflammatory are dose-dependent.