摘要
[目的]优化零陵香水溶性组分中多糖的最佳提取工艺,研究其抗炎效果。[方法1应用DesignExpert软件响应面法(RSM)对零陵香多糖的提取工艺进行优化,以最佳提取工艺提取零陵香多糖,并进行抗炎实验。[结果]零陵香多糖浸提的最佳工艺条件为:温度98℃,时间20min,液料比30mL/g,实际最大提取率为1.28%,与预期的1.34%拟合度较高。零陵香多糖在0.75~3.00g/kg(生药)浓度范围具有抗炎能力。[结论]响应面法可应用于零陵香多糖提取的工艺优化;其抗炎效果与剂量成呈正相关I生。
[Objective] To optimize the excellent extracted technique of water-soluble polysaccharide of Lysimachiafoenum-graecum Hance (LP) by RSM and study its anti-inflammatory actions. [Methods] Using Design-Expert (RSM) the extract technique were optimized and the PL was extracted with the obtained technique. The anti-inflammatory actions of LP were observed. [Results] The best factors expected (yield 1.34%) were 98 ℃, 20 min, 30 mL/g, and the real yield was 1.28%. LP had anti-inflammatory actions within the range of 0.75-3.00 g/kg. [Conclusion] RSM can be used for LP extraction and the actions of anti-inflammatory are dose-dependent.
出处
《天津中医药》
CAS
2013年第4期235-238,共4页
Tianjin Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81274042)
关键词
响应面法
零陵香
多糖
抗炎
中药组分
RSM
Lysimachiafoenum-graecum Hance
polysaccharide
anti-inflammatory
active components of traditional Chinese medicine