胃和冲剂Ⅱ号对慢性萎缩性胃炎大鼠胃黏膜三叶因子1和CD34表达的影响

Effect of weihe granule Ⅱ on expression level of trefoil factor 1 and CD34 in rats with chronic atrophic gastritis

目的观察胃和冲剂Ⅱ号对慢性萎缩性胃炎（CAG）大鼠胃黏膜三叶因子1（TFF1）和CD34表达的影响，探讨胃和冲剂Ⅱ号治疗CAG的作用机制。方法将72只雄性Wistar大鼠随机分为正常对照组（12只）和造模组（60只）。除正常对照组外，其余各组大鼠均使用综合造模法成功复制CAG动物模型（为确定造模成功，颈椎脱臼法处死9只大鼠）。然后将53只CAG大鼠随机分为5组，分别为模型组（10只）、维酶素阳性对照组（11只）及胃和冲剂低、中、高剂量组（10只，11只，11只）。治疗60d后，取各大鼠相同部位全层胃壁组织，采用免疫组化法分别检测胃黏膜组织的TFF1和CD34的表达水平。结果TFF1在胃和冲剂高剂量组中的表达值（3．4±1．4），高于模型组（1．5±0．8）和维酶素组（1．7±0．9）（P〈0．01）；胃和冲剂高剂量组微血管密度值为（7．5±1．5），低于模型组（12．5±2．5）和维酶素阳性对照组（11．6±2．1），差异有统计学意义（P〈0．01）。结论胃和冲剂Ⅱ号可能通过提高胃黏膜组织的TFF1表达、降低CD34的表达，加快胃黏膜细胞的修复和抑制过度增殖，从而对CAG起到治疗作用并可预防癌变。

Objective To explore the treatment effect of Weihe granule Ⅱ on chronic atrophic gastritis （CAG） by analyzing the expression level of trefoil factor 1 （ TFF1 ） and CD34 in gastric mucosal of model rats of chronic atrophic gastritis（CAG）. Methods Seventy-two male Wistar rats were randomly divided into two groups, control group （n = 12） and model group （n = 60）. The rat model of CAG in model group was made by using the comprehensive modeling method. Then, all rats in model group were assigned into five sub-groups, namely model sub-group, vitacoenzyme control sub-group, low-dose, mediate-dose and high-dose Weihe granule Ⅱ sub-groups. Then all rats in each group were subject to the corresponding treatments for 60 days. The expression level of TFF1 and CD34 in the same gastric tissue of each rat were examined and compared with each groups. Results The expession of TFF1 in the high-dose weihe granule Ⅱ sub-group [ （3.4 ± 1.4） ] was higher than that in model sub- group [ （ 1.5 ± 0.8） J and control sub-group [ （ 1.7 ± 0.9） ] （P 〈 0.01 ） ; and the microvascular density value, which were marked by CD34, was 7.5 -± 1.5 in the high-dose Weihe granule Ⅱ sub-group which was lower than those in model sub-group and vitacoenzyme control sub-group （P 〈 0.01 ）. Conclusions Weihe granule Ⅱ can increase the expression level of TFF1 and decrease the expression level of CD34 in gastric mucosal, it can repair the gastric mucosal cells and inhibit the excessive proliferation, cure CAG and prevent cancer.