云南省1997-2010年新型肠道病毒监测研究

Sequencing analysis to identify newer enteroviruses isolated from 1997 to 2010 in Yunnan province of China

目的对云南省1997-2010年分离的新型肠道病毒进行基因测序定型并对EV75、EV96的基因特征进行分析。方法按文献报道的方法对云南省1997-2010年急性迟缓性麻痹病例(AFP)及2007-2010年健康人群中分离的"不能定型的肠道病毒(untypable enteroviruses)"进行RT-PCR和VP1区测序定型。结果共检测到7种新型肠道病毒,计25株。其中,A组病毒1个血清型(EV76,2株),B组病毒5个血清型(EV73,3株;EV75,3株;EV80,2株;EV81,1株;EV83,3株),C组病毒1个血清型(EV96,11株)。7种新型肠道病毒中,EV96分离年份最广:在AFP病例中于1998年、1999年、2000年和2004年分离到,在健康人群中于2010年分离到。结论新型肠道病毒在云南省分布广泛,尤其以EV96型分离数最多,分离年份最广。EV75、EV96具有基因多样性特点,但新型肠道病毒与疾病的关系值得进一步研究。

Objective To determine the serotypes of newer human enteroviruses （EV） isolated in Yunnan, China from 1997 to 2010. Methods Newer EV strains were isolated from patients of acute flaccid paralysis （AFP） and healthy individuals. RT-PCR was used to amplify the VP1 region of enterovirus and then the se- quences were compared with reference sequences. Results A total of 7 serotypes of newer EV （25 strains） were identified by sequencing analysis of the VP1 gene. One serotype was grouped as HEV-A （EV76, 2 strains）; 5 serotypes were categorized as HEV-B （3 strains each of EV73 and EV75, 2 strains of EV80, 1 strain of EV81, 3 strains of EV83）; 1 serotype was classified as HEV-C （EV96, 11 strains）. Conclu- sions Newer EVs in Yunnan of China are widely distributed with EV96 as the most popular strain. EV75 and EV96 showed multiple genetic variances but the clinical significance of these newer EVs needs to be further studied.