视力检查遮眼板细菌学监测及3种消毒剂消毒效果对比

Bacteriological monitoring of eye cover for visual test and effectiveness of disinfection with three disinfectants

目的了解视力检查遮眼板的带菌情况及3种消毒剂消毒效果比较,探讨适宜的消毒方法。方法在消毒前使用500mg/L含氯消毒剂、75%乙醇、异丙醇复合消毒湿巾3种不同方法对遮眼板消毒处理后即刻采样、使用5、10人次后分别采样,进行细菌学检测,计数菌落数并进行细菌鉴定,检测结果进行统计分析。结果未经消毒的遮眼板带菌率100.00%,主要病原菌为金黄色葡萄球菌、铜绿假单胞菌及变形菌属;分别用500mg/L含氯消毒剂、75%乙醇、异丙醇复合消毒湿巾3种方法消毒后即刻采样结果,细菌总数均减少,杀菌率分别为99.46%、99.46%、99.89%,与消毒前相比,差异有统计学意义(P<0.01);使用5、10人次后采样检测结果显示,异丙醇复合消毒湿巾的消毒持续效果优于含氯消毒剂及乙醇(P<0.01或P<0.05)。结论视力检查遮眼板带菌情况严重,应进行消毒处理,异丙醇复合消毒湿巾、500mg/L含氯消毒剂消毒效果优于75%乙醇。

OBJECTIVE To understand the microbiological contamination of eye cover used for visual test and to discuss appropriate disinfection ways.METHODS The eye covers were sampled instantly after being disinfected with three disinfectants including 500 mg/L chlorine-containing disinfectant,75% ethanol,and isopropanol composite disinfectant wipes,and were sampled after the eye covers were respectively used for 5 case-times and 10 case-times,the bacteriology test was performed,the bacterial colony counts were calculated,the bacteria were identified,and the statistical analysis of the test result was performed.RESULTS The microbial contamination rate was 100.0% before the disinfection,Staphylococcus aureus,Pseudomonas aeruginosa and Proteus were the main species of pathogens.After the being respectively disinfected by 500 mg/L chlorine-containing disinfectant,75% ethanol,and isopropanol composite disinfectant wipes,the total bacterial colony counts were significantly decreased,and the germicidal rates were 99.46%,99.46%,and 99.89%,respectively,as compared with those before the disinfection,the difference was statistically significant（P0.01）.The test result for the sampling after being used for 5 case-times and 10 case-times indicated that the isopropanol composite disinfectant wipes was superior to the 500 mg/L chlorine-containing disinfectant and 75% ethanol in the continuous disinfection effect（P0.01 or P0.05）.CONCLUSION The bacterial contamination of the eye covers is so serious that it is necessary to be disinfected,the 500 mg/L chlorine-containing disinfectant and the isopropanol composite disinfectant wipes are superior to 75% ethanol in the disinfection effect.