宫颈分泌物支原体属与衣原体属感染检测及药敏分析

Detection of Mycoplasmaand Chlamydiain cervical secretions and analysis of antibiotics susceptibility

目的探讨1286例女性宫颈分泌物支原体属、衣原体属感染状况,分析其耐药性,为临床治疗提供依据。方法选择2011年1月-2012年12月在医院就诊的1286例女性患者,取宫颈分泌物,采用培养法检测支原属体并进行药敏试验,采用单克隆抗体胶体金法对衣原体属进行检测。结果 1286例女性患者中,沙眼衣原体(Ct)阳性106例,阳性率8.2%;支原体属阳性568例,阳性率44.1%,其中人支原体(Mh)阳性42例,阳性率3.2%、解脲脲支原体(Uu)阳性368例,阳性率28.6%,Uu+Mh阳性158例,阳性率12.3%;Uu+Ct混合感染41例,阳性率3.2%;支原体属药敏试验结果显示,单纯Uu和Mh红霉素均保持比较高的耐药性,两者混合感染的耐药性加强。结论对女性生殖道疾病患者进行支原体属、衣原体属检查,并进行药敏试验,可以有效指导临床用药。

OBJECTIVE To discuss the status of infections of Mycoplasma and Chlamydia isolated from the cervical secretions of 1286 female patients and analyze the drug resistance so as to guide the clinical treatment.METHODS A total of 1286 women who were treated in the hospital from Jan 2011 to Dec 2012 were selected,and the cervical secretions were sampled.Mycoplasma were detected by the culture method and the drug susceptibility testing was performed,and Chlamydia was detected by using monoclonal antibody colloidal gold method.RESULTS Of totally 1286 cases of female patients investigated,there were 106（8.2%） cases with Chlamydia trachomatis（Ct） detected positive and 568（44.1%） cases with Mycoplasma tested positive,including 42（3.2%） cases with Mh,368（28.6%） cases with Uu,158（12.3%） cases with Uu＋Ct,and 41（3.2%） cases with Uu＋Ct.The result of drug susceptibility testing indicated that the single Uu or Mh remained high drug resistance to erythromycin,and the drug resistance was increased when any of two was mixed.CONCLUSION To perform the detection of Mycoplasma and Chlamydia for the female patients with genital tract diseases and conduct the drug susceptibility testing can effectively guide the clinical medication.