丙戊酸钠促进猪再克隆胚胎体外发育

Vaproic acid improved the in vitro developmental competency of porcine recloned embryos

对VPA处理的最佳处理时间和处理浓度进行探索,希望通过VPA处理来改善再克隆胚胎的发育能力。结果表明,使用1mmol/L VPA处理16h,可以获得较高的囊胚形成率。然后通过免疫荧光检测发现,囊胚时期acH3K9的乙酰化水平在处理组中高于非处理组。多能性因子Oct4、Nanog、Sox2和Klf4的实时定量检测结果则显示,它们之间的表达水平同样差异不显著。结果表明VPA能够显著提高猪再克隆胚胎体内发育能力。

In the present study, to promote the developmental competency of porcine recloned embryos through vaproic acid （VPA） treatment, the treated time and concentration for VPA was firstly exploited. The results showed that the highest blastocyst formation rate could be harvested when treated with 1 mmol/L VPA for 16 h. Through immunostaining,it showed that the fluorescent intensity of acH3K9 was higher in the VPA treated group at blastocyst stage,compared with control. There were no significantly differences between the treated and non-treated group in the gene expression level of Oct4,Nanog, Sox2 and Klf4, as was revealed by Real-time PCR analysis. These results provide evidences that the in vitro developmental competency of porcine recloned embryos could be improved with VPA treatment.