新疆产阿育魏实挥发油及不同极性部位抗氧化活性研究

Research on Antioxidant Activity of Different Polar Fractions and Volatile Oil of Trachyspermum ammi L.Sprague in Xinjiang

采用2,2'连氨-双-(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)和二苯代苦味酰基自由基(DPPH),还原能力法对阿育魏实抗氧化活性进行研究,并测定了各样品中总酚和总黄酮含量。结果表明:阿育魏实挥发油和甲醇提取物具有明显的抗氧化能力,清除DPPH.IC50为0.22mg/mL和0.16mg/mL,ABTS.IC50为3.82mg/mL和3.20mg/mL。在3mg/mL时,挥发油和不同溶剂提取物中甲醇提取物表现出较好的还原能力。挥发油和提取物抗氧化活性与酚类和黄酮类化合物具有很大相关性。

To study the antioxidant activity of essential oil （EO） and different solvents that extracted from Trachyspermurn ammi L. Sprague （ T. ammi）. The antioxidant activities of T. ammi EO are evaluated based on ABTS and DPPH by Capacity Reducing Method. The total contents phenol and flavonoid of volatile oil and solvents are also determined. The EO and methanol extract （ME） have good antioxidant activities on ABTS and DPPH radicals, the scavenging activities of DPPH with an ICs0 are 0.22, 0. 16 mg/mL, while those of ABTS with an ICs0 are 3.82, 3.20 mg/mL respectively. The methanol extract has a good reducing capacity at 3 mg/mL in different solvent extracts. There is a positive correlation between antioxidant activity and amount of phenolic and flavonoids compounds of the EO and extracts.