高聚物萃取分光光度法测定茶多酚含量的研究

Study on polymer extraction and spectrophotometer determine of tea-polyphenols

利用水溶性高聚物的水溶液在无机盐存在下可分成两相的特点，将茶多酚转至高聚物相，加入三羟甲基氨基甲烷，调节溶液pH8，以酒石酸亚铁为显色剂，用分光光度法测定茶饮料中的茶多酚含量。结果显示，茶多酚在0．004-0.4mmol·L^-1浓度范围内具有良好的线性关系。其相关系数R^2=0．9999，加标回收率为92．4％～103．5％，该方法检测快速、准确、无毒，而且易于操作。

Make use of the trait of aqueous polymer could separated two phases with solution in the presence of inorganic salt,tae-polyphenol was transferred polymer phase. In an Tris buffer medium of pH8,the content of tae-polyphenol was determined with Spectrophotometer. Good linear relationships was obtained in the range of 0.004N0.4mmol·L^-1 of tae-polyphenol per milliliter of solution（R^2=0.9999）,and recoveries were found in the range of 92.4%-103.5%. The method was quick,accurate and handled easily.