水-酶连续提取百合非淀粉多糖工艺研究

Study on successive extraction of non-starch polysaccharides from bulb of tiger lily(Lilium lancifolium Thunb.) with water and pectinase

研究了热水和果胶酶连续提取百合鳞茎中非淀粉多糖的工艺条件。首先利用Box-Behnken实验设计及响应面分析确定热水提取百合非淀粉多糖的条件;再经单因素实验与正交实验优化果胶酶提取水提百合渣中非淀粉多糖的工艺参数。结果表明,水-果胶酶连续提取百合非淀粉多糖的最佳工艺条件为:温度62℃、液料比19∶1,水提4h后,剩余残渣中加入果胶酶(酶添加量1800U/g)于温度30℃、pH5.5的条件下酶解20min。在此条件下,百合非淀粉多糖的得率为8.63%。

Extraction conditions of lily non-starch polysaccharides（NSP） with water and pectinase were optimized in this study. Firstly,the extraction condition of lily NSP with water was determined using Box-Behnken design and analyzed by response surface methodology（RSM）. Subsequently, NSP from the lily bulb residue left by hot water was extracted with pectinase,and the results of extraction condition optimization was obtained by the orthogonal optimization experimental system. The result indicated that the optimum condition as follows： extraction temperature 62℃ ,extraction time 4h and ratio of water to raw material 19：1 ,and then the lily bulb residue left by hot water was extracted with pectinase when the pH value,the concentration of enzyme,the extraction time and the temperature were 5.5,1800U/g,20min and 30℃,respectively. Under this condition,the NSP yield was 8.63%.