百合花瓣酚类物质及其抗氧化活性的分析

Phenolic Contents and Antioxidant Activity of Lily Flower Petals

目的:研究3个栽培百合品种("索邦"、"西伯利亚"及"黄天霸")和岷江百合花瓣的酚类物质及抗氧化活性。方法:测定4类百合花瓣的酚类物质及15种单体酚含量,并通过测定5个抗氧化指标(ABTS+.、DPPH自由基、羟自由基(.OH)的清除能力,铜离子还原能力以及金属螯合能力对其抗氧化活性进行评价。结果:花瓣酚类物质含量由高到低次序为:"索邦">"西伯利亚">"黄天霸">岷江百合。其中单体酚的种类也有所不同,但均含有儿茶素、飞燕草素、表儿茶素、二氢杨梅酮、芦丁、香豆酸、二氢槲皮素、山奈酚。4类百合花瓣酚类物质对ABTS+.、DPPH自由基、.OH均有较好的清除效果,对铜离子均有较强的还原能力以及良好的金属螯合能力。结论:4类百合花瓣均适合作为天然抗氧化材料进行开发应用。

The contents of phenols including 15 phenol monomers and antioxidant activity of four cultivated varieties of liliy flower petals were tested. The antioxidant activity of petal extracts was evaluated by measuring scavenging ability against ABTS＋·, DPPH and hydroxyl free radicals, reducing power and metal-chelating ability. Flower petals of “Sorbonne” was found to contain the highest phenol content followed by “Siberia”, “Manissa” and Lilium regula. Different types of polyphenol monomers were found in the studied varieties of lily petals and catechin, epicatechin, pcoumaricacid, delphinidin, rutin, dihydromyricetin, dihydroquercetin and kaempferol were simultaneously present in them. In addition, phenols from 4 varieties showed strong radical scavenging activity against ABTS＋·, DPPH and hydroxyl free radicals, copper ion reducing power and metal-chelating capacity. Our results indicate that these selected lilies can be developed and utilized in practice as promising sources of natural antioxidants.