摘要
采用真菌诱导子提高纳他霉素的产量,通过初筛,获得具有正向调节作用的真菌诱导子;采用Box-Behnken试验设计建立数学模型,进行响应面分析优化,确定诱导条件。结果表明:黑曲霉代谢产物诱导子能够有效提高纳他霉素的产量;质量浓度为122μg/mL的黑曲霉代谢产物诱导子在纳他霉素发酵18.3h后加入,诱导78.5h时,发酵液中纳他霉素的发酵产量可达到2.89g/L,为空白对照组的1.68倍。
Preliminary screening was carried out to obtain fungal elicitor that induces natamycin production by Streptomyces chattanovgensis. The optimal induction conditions were established by mathematical modelling mathematical modelling based on Box-Behnken experimental design and response surface analysis. The potent inducing effect of the fungal elicitors on natamycin production was confirmed. When fungal elicitor from Aspergillus niger was added to a final concentration of 122 μg/mL after 18.3 h natamycin fermentation and incubated for 78.5 h, the observed average yield of natamycin in fermentation broth was 2.89 g/L, 1.68 times higher than the blank control.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第7期196-200,共5页
Food Science
基金
辽宁省教育厅科研项目(L2012248)
关键词
真菌诱导子
发酵
纳他霉素
响应面
fungal elicitor
fermentation
natamycin
response surface analysis
