葡萄籽原花青素对Ⅱ型糖尿病大鼠视网膜病变的影响

Effect of Grape Seed Proanthocyanidin Extract (GSPE) on Type Ⅱ Diabetic Retinopathy in Rats

目的:观察葡萄籽原花青素(GSPE)对Ⅱ型糖尿病大鼠视网膜病变的影响。方法:雄性SD大鼠高脂饲料喂养4周后,腹腔注射链脲佐菌素(STZ)造成糖尿病模型,成模后随机分为糖尿病模型组和原花青素低、中、高剂量组;以基础饲料喂养为正常对照组。原花青素低、中、高剂量组分别为125、250、500mg/(kg.d)(以体质量计),通过每天灌胃水溶液的方式进行干预,糖尿病模型组和正常对照组每天以蒸馏水灌胃。连续饲养16周后,测定其体质量、空腹血糖、血清糖基化终末产物(AGEs)、细胞间黏附分子(ICAM-1)、血管间黏附分子(VCAM-1),镜下观察视网膜的变化,免疫组化法测定视网膜中糖基化终产物受体(RAGE)的表达。结果:GSPE干预组血糖较糖尿病模型组有一定下降,但无显著性差异(P>0.05);GSPE高剂量组可以有效降低血清中AGEs水平(P<0.05);GSPE各干预组可以有效降低血清中ICAM-1水平(P<0.05);GSPE低剂量和高剂量组可以有效降低血清中VCAM-1水平(P<0.05);从组织学上,GSPE可以减轻视网膜损伤,保持视网膜各层完整;GSPE能降低RAGE在视网膜中的表达。结论:葡萄籽原花青素可以有效改善Ⅱ型糖尿病大鼠视网膜病变。

Objective： To investigate the effect of grape seed proanthocyanidin extract （GSPE） on type Ⅱ diabetic retinopathy in rats. Methods： To establish type Ⅱ diabetic rat models, male SD rats fed high fat diet for 4 weeks were injected with streptozotocin （STZ, 0.01 mol/L, 30 mg/（kg？d））. The diabetic rats were randomly divided into diabetes-vehicle group （DM control group）, three GSPE-treated groups. Normal rats were enrolled as normal control group. The low, medium and high-dose GSPE-treated groups were administrated at 125, 250 mg/（kg？d） and 500 mg/（kg？d）, respectively. DM control group and normal control group were given equal volume of distilled water. After 16 weeks, body weight, fasting plasma glucose （FPG）, advanced glycation endproducts （AGEs）, ICAM-1 and VCAM-1 were measured. HE stain retina paraffin section was examined under a microscope. Receptor for RAGEs was detected by SP immunohistochemical method. Results： There was no difference （P 〉 0.05） between DM control group and GSPE-treated groups. Compared with DM control group, AGEs in GSPE high dose group were decreased （P 〈 0.05）, ICAM-1 （all GSPE-treated groups） and VCAM-1 （GSPE low and high dose groups） were decreased （P 〈 0.05）. The retina of GSPE-treated groups had fewer damaged areas and lower RAGE expression in histology. Conclusion： GSPE has a positive effect on type Ⅱ diabetic retinopathy in rats.