酪蛋白糖巨肽对大鼠肠黏膜屏障的保护作用

Protective Role of CGMP in Intestinal Mucosal Barrier Function in Rats

目的:探讨不同剂量酪蛋白糖巨肽(CGMP)干预结直肠癌大鼠过程中,肠三叶因子(ITF/TFF3)和血管内皮生长因子(VEGF)表达的变化情况,从而推断CGMP对大鼠肠黏膜屏障2种关键因子表达的影响。方法:40只Wistar雄性大鼠随机分为正常对照组、模型组、低剂量CGMP组、中剂量CGMP组和高剂量CGMP组。除正常对照组外,对每只大鼠每周腹腔注射1,2-二甲基肼二盐酸盐(DMH),剂量为30mg/kg,同时,3个剂量CGMP组注射DMH的同时每天灌胃CGMP,剂量分别为10、50、100mg/(kg.d)。8周后,处死大鼠,取结肠组织,采用免疫组化方法检测TFF3和VEGF的表达情况。结果:HE染色结果表明CGMP可以改善大鼠肠黏膜的损伤;免疫组化结果证实了CGMP可以显著降低大鼠肠黏膜VEGF的表达,而对TFF3的表达没有影响。结论:CGMP具有修复大鼠肠黏膜屏障损伤的功能。

Objective： To explore the effect of caseino glycomacropeptide（CGMP） intervention on the expression of trefoil factor and vascular endothelial growth factor in the colon and deduce the effect of CGMP on key factors of intestinal mucosal barrier function in rats with colorectal cancer. Methods： A total of 40 Wistar rats were randomly divided into five groups including control group, model group, low-dose CGMP group, middle-dose CGMP group and high-dose CGMP group. Except the control group, all the rats were intraperitoneally injected with dimethyl hydrazine at a dose of 30 mg/kg. At the same time, the rats from CGMP groups were injected with 1,2-dimethylhydrazine dihydrochloride and administered with CGMP at dosages of 10, 50, 100 mg/（kg？d）, respectively. After eight weeks, all the rats were killed to harvest colon tissues for determination of the expression of ITF and VEGF by immunohistochemical method. Results： HE staining revealed that CGMP might regulate damaged intestinal barrier function in rats. Meanwhile, IHC staining confirmed that CGMP could reduce the expression of VEGF, but had no effect on the expression of TFF3. Conclusion： CGMP may repair damaged intestinal barrier function in rats.