气相色谱-质谱法同时测定皮革制品中9种酚类化合物

Simultaneous Determination of 9 Phenols in Leather Products by Gas Chromatography-Mass Spectrometry

建立了气相色谱-质谱法(GC-MS)同时测定皮革制品中9种酚类化合物的分析方法。样品用丙酮溶液超声提取,将提取溶液与氢氧化钾溶液混合乙酸酐衍生化反应。衍生产物经正己烷提取后,采用GC-MS进行分析,内标法定量。结果表明,方法的线性范围为50～500ng/mL,相关系数≥0.9990;方法的定量限为0.05mg/kg,平均回收率为87.1%～114.3%,相对标准偏差小于8.5%。该方法准确、灵敏,可用于皮革制品中9种酚类化合物的分析。

A method for the determination of 9 phenols in leather products was developed by gas chromatography-mass spectrometry （GC-MS）. The sample was extracted with acetone by ultrasonicationl the extract was mixed with potassium hydroxide and then reacted with acetic anhydride. The derivative was extracted with hexane and determined by GC-MS, and was quantified by internal standard method. The linear calibration curve was obtained in the range of 50-500 ng/mL with a correlation coefficient above 0. 9990. The limit of quantification was 0. 05 mg/kg and the average recoveries were in the range of 87.1%-114.3 % for 9 phenols with the relative standard deviations below 8.5 %. This method is accurate and sensitive, and it is suitable for the analysis of phenols in leather products.