中性蛋白酶对核桃蛋白水解条件的优化研究

Optimization of hydrolysis conditions of walnut protein by neutrase protease

重点研究中性蛋白酶对核桃蛋白的水解条件,采用响应面分析方法对核桃蛋白的水解条件进行优化,得到各因素的最佳水平值。中性蛋白酶水解核桃蛋白的最适反应条件为:水解时间3.5 h,底物质量浓度2.9 g/100 mL,加酶量4 364.1 U/g(占底物质量),温度48.4℃,pH 7.0。在此条件下,水解度达到12.8%。

Hydrolysis conditions of walnut protein by neutrase protease were studied and optimized by re- sponse surface methodology. The optimal reaction conditions were as follows ： reaction time 3.5 h, substrate mass concentration 2.9 g/100 mL, reaction temperature 48.4 ℃, pH 7.0 and enzyme dosage 4 364.1 U/g（based on the mass of substrate）. Under these conditions, the degree of hydrolysis was 12.8%.