微生物Mucor sp.转化肉桂酸生成苯乙酮降解途径分析

Analysis of Degradation Pathways from Cinnamic Acid to Acetophenone by Mucor sp.

利用GC-MS检测Mucor sp.转化肉桂酸的产物,对微生物Mucor sp.降解肉桂酸的途径进行了分析。β-苯乙醇首次作为微生物Mucor sp.转化肉桂酸生成苯乙酮的中间体在产物中被检测到。Mucor sp.降解肉桂酸的途径是:在肉桂酸的侧链双键上先发生环氧化反应得到3-苯基-2,3-环氧丙酸,环氧化合物经脱羧、还原开环得到中间体β-苯乙醇,β-苯乙醇经羟基转移和进一步催化氧化生成苯乙酮。

The metabolic pathway for the degradation of cinnamic acid with Mucor sp. was studied by analyzing metabolites using GC-MS. /3-Phenethyl alcohol, for the first time, was detected as an intermediate in transforming cinnamic acid to acetophenone by Mucor sp.. The metabolic pathway of cinnamie acid was supposed： epoxidation reaction occurred on the double bond of cinnamic acid side chain to generate ethyl 2,3-epoxy-β-phenylpropionic acid, the epoxide was converted to β-phenethyl alcohol via decarboxylation and ring-opening reaction, and, lastly, acetophenone was produced from fl- phenethyl alcohol via hydroxyl migration and oxidation reaction.