镁补充对2型糖尿病大鼠红细胞胰岛素受体亲和力的影响

Influence of magnesium supplementation on insulin receptor affinity in erythrocytes of type 2 diabetes rats

目的研究镁补充对2型糖尿病大鼠红细胞胰岛素受体亲和力的影响。方法将高脂饲料喂饲加链脲佐菌素腹腔注射诱发的2型糖尿病大鼠随机分成糖尿病对照及高、中、低剂量组,饲料中分别加入氧化镁(以镁计)0、2000、1000、200mg/kg,正常对照组喂饲普通饲料,共喂养4周。检测红细胞胰岛素受体结合常数、结合容量、受体数目、空腹血糖及空腹胰岛素等指标,计算胰岛素抵抗指数与敏感指数。结果高剂量组高亲和力胰岛素受体结合常数、结合容量和受体数目分别为(1.24±0.47)×109L/mol、(1.26±0.53)×1014/L和80.23±0.47,均高于糖尿病对照组;胰岛素抵抗指数较糖尿病对照组降低而胰岛素敏感指数升高。结论镁补充能提高2型糖尿病大鼠红细胞胰岛素受体亲和力,改善胰岛素抵抗。

Objective To observe the influence of oral magnesium supplementation on insulin receptor affinity in erythrocytes of type 2 diabetes rats. Method Type 2 diabetes rats were induced by high-fat-diet and intraperitoneal injection of streptozocin, and divided into 4 groups. Magnesium was supplemented in high-fat-diet at the dose of 2000, 1000, 200 and 0 mg/kg,respectively. Normal control rats were fed with ordinary diet. Rats were killed after four weeks. Erythrocytes insulin receptor number, combination constants, combined capacity, fasting insulin level and fasting glucose levels were measured, and insulin resistance index and insulin sensitivity index were calculated. Results High affinity insulin receptor combination constant, combined capacity and receptor number in high dosage group were（ 1.24 ＋0.47） x 109L/tool, （ 1.26 ＋0.53 ） x 1014/L and 80.23 ＋ 0.47 respectively, significantly higher than diabetes control group. Insulin resistance index decreased and insulin sensitivity index increased in high dosage group than diabetes control. Conclusion Magnesium supplementation could improve erythrocyte insulin receptor affinity and improve insulin resistance in type 2 diabetic rats.