饮水氟暴露对成年男性性激素结合球蛋白及睾酮水平的影响

Influence of water fluoride exposure on sex hormone binding globulin and testosterone in adult male

目的探讨饮水氟暴露对成年男性性激素结合球蛋白(SHBG)及睾酮水平的影响。方法依据水氟检测结果,在河南省通许县随机选择3个村庄作为调查点,分别为高氟村、改水村和对照村;整群抽取调查区本地生长的18～50岁成年男性,收集空腹静脉血和晨尿。应用氟离子选择电极法测定饮用水和尿中氟的含量;用酶联免疫吸附法(ELISA)测定其血清中SHBG水平,化学发光法测定血清睾酮的含量。结果血清SHBG浓度分别为对照组47.85nmol/L,改水组31.37nmol/L,高氟组24.52nmol/L,3组比较差异均有显著性(P<0.05)。血清睾酮水平分别为对照组3.69ng/ml,改水组4.61ng/ml,高氟组4.83ng/ml,高氟与对照组比较,差异有显著性(P<0.05)。高氟组血清SHBG水平与睾酮呈正相关关系(r=0.230,P=0.049),而在改水组和对照组中未观察到相关关系。结论长期氟暴露可影响成年男性血清SHBG水平和睾酮水平,并可能存在剂量-效应关系。

Objective To explore the influence of water fluoride exposure on sex hormone binding globulin （SHBG） and testosterone in adult male. Methods Cross- sectional study was conducted in three villages of Tongxu county including high fluoride group （HFG） , defluoridation project group （DFPG） and control group （CG） based on the fluoride concentration in drinking water. Adult male who were born and raised in the village and aged 18 -50 years old were recruited using cluster sampling. Fasting blood and morning urine samples were collected. The fluoride levels in drinking water and urine were detected by fluoride-ion selective electrode method. Serum SHBG level was determined using enzyme-linked immunosorbent assay （ ELISA ）. The chemical luminescence immune analysis method was used to detect serum testosterone content. Results Serum SHBG level was 47.85nmol/L in CG, 31.37nmol/L in DFPG and24.52nmol/L in HFG respectively. There were significant difference among of three groups （P 〈 0.05 ）. Serum testosterone level was 3.69ng/ml in CG, 4.61ng/ml in DFPG and 4.83ng/ml in HFG respectively. Serum testosterone level in HFG was significantly higher than that in CG （P 〈 0. 05）. Serum SHBG level in HFG has positive correlation with serum testosterone （r = 0. 230, P = 0. 049）, which has not been observed in DFPG and CG. Conclusions Long-time fluorine exposure may affect serum SHBG and testosterone level in adult male.