表面活性剂对二氧化氯溶液杀菌效果增效作用研究

Study on synergistic effect of bactericidal effect of chlorine dioxide solution by surfactant

目的研究三种表面活性剂双子氟碳、全氟壬烯氧基苯磺酸钠及十二烷基硫酸钠对二氧化氯溶液在物体表面杀菌效果的影响。方法以纯二氧化氯溶液作为基准消毒液,依据《消毒技术规范》(2002年版)分别采用载体定量杀菌试验及现场模拟试验进行研究。结果载体定量杀菌试验表明,当消毒液中双子氟碳、十二烷基硫酸钠及全氟壬烯氧基苯磺酸钠加入量分别为60、60和40mg/L时,二氧化氯消毒液浓度为50mg/L,对受污染菌片上的金黄色葡萄球菌作用10min,各次的杀灭对数值均>3;当消毒液中双子氟碳、十二烷基硫酸钠及全氟壬烯氧基苯磺酸钠加入量为60mg/L时,二氧化氯消毒液浓度为20mg/L,对大肠杆菌作用10min,各次的杀灭对数值均>3。杀菌效果好于单独使用二氧化氯溶液的杀菌效果。现场模拟试验表明,消毒液中二氧化氯浓度为20mg/L,全氟壬烯氧基苯磺酸钠浓度为40mg/L,消毒液对物体表面的大肠杆菌作用15min,各次的杀灭对数值均>3。结论表面活性剂对二氧化氯溶液杀菌效果有一定的增效作用。

Objective To study the effect of gemini fluorocarbon, sodium p- perfluorous nonenoxybenzene sulfonate and sodium dodeeyl sulfate on the chlorine dioxide solution sterilization to object surface. Methods Pure chlorine dioxide solution as the reference disinfectant, carrier quantitative bactericidal test and simulated test on-site were used to carry out laboratory observation according to The disinfection technical specifications （2002）. Results Carrier quantitative bactericidal test showed that the addition dosage of gemini fluoronates, sodium dodecyl sulfate surfactant and perfluorinated the nonene oxy i benzene sulfonate in disinfectant solution were 60, 60 and 40mg/L respectively, the killing log value of Staphylococcus aureus exposed to the disinfectant solution containing chlorine dioxide 50mg/L for 10rain were all more than 3; and the addition dosage of gemini fluorinates, sodium dodecyl sulfate and perfluorinated the nonene oxy benzenesulfonate in disinfectant solution were 60mg/L, the killing log value of Escherichia coli exposed to the disinfectant solution containing chlorine dixoxide 20mg/L for 10min were all more than 3. The bactericidal effect of the mixture use of surfactant and chlorine dioxide was better than the single use of chlorine dioxide. The simulated test on-site showed that the killing log value of Escherichia coli exposed to the disinfectant solution containing perfluorinated the nonene oxy benzene sulfonate 40mg/L and chlorine dioxide 20mg/L for 15min was more than 3. Conclusion Surface active agent on germicidal efficacy of chlorine dioxide solution had synergistic action.