烟草叶片组织结构的扫描电镜观察方法

Observation of the structure of tobacco leaf tissue with SEM

将烟草叶片经过固定、水解、剥离、降解、浸泡、置换、干燥、喷金等处理 ,成功地制备出了烟草叶片各层组织的扫描电镜样品 ;首次获得烟草叶片各组织层的立体结构图像 ,通过比较 ,发现不同部位叶片内部组织结构存在某些规律差异。为研究烟草叶片各组织层中细胞分布和生长发育规律提供了一条新的途径。

Scanning electron microscope samples of the epidemis, palisade tissue layer, spongy tissue layer of tobacco leaf are successfully preparated with the method proposed in this paper. The structure of the tissue layers of tobacco leaf is observed with SEM.