川渝地区三年桐核心种质的初步构建

Establishment of Key Germ Plasm of Vernicia fordii in Sichuan and Chongqing Areas

对川渝地区300份三年桐资源按照种群的方式分成12组,然后运用分组聚类法分别按30%、25%、20%、15%、10%的比例抽取5个候选核心种质,比较Shannon表型遗传多样性指数HI并与总体种质进行差异显著性检验,结果显示20%为最佳取样比例,从而确定核心种质60份。通过对核心种质进行符合性检验,平均值符合率、标准差平均符合率和变异系数平均符合率分别为98.07%、94.12%和95.70%。结果表明,核心种质基本上可以较好的代表总体种质的遗传变异。

The 300 parts of VF germplasm materials in Sichuan and Chongqing areas were divided into 12 groups according to the population way,then by group clustering method,5 candidates were collected （in the proportion of 30% ,25% ,20%, 15% and 10% ）and their genetic diversity indexes were compared and the significance of their difference was tested. Finally 60 parts of key germ plasm were established un- der 20% of the sampling rate. Through testing the characteristic value coincidence of key germ plasma, the mean compliance rate, the standard deviation rate and the coefficient of variation were 98.07%, 94. 12% and 95.70% ,which showed that the primary key germ plasm was able to represent genetic vari-ation of the total germplasm.