牡丹皮HPLC指纹图谱研究

HPLC fingerprint of Moutan Cortex

目的建立牡丹皮的HPLC指纹图谱。方法色谱柱为Dikma Diamonsil C18色谱柱(250mm×4.6mm,5μm);以乙腈-水(含0.085%磷酸)为流动相梯度洗脱;柱温35℃;体积流量1mL/min;检测波长254nm(0～21.5min),230nm(21.5～45min),254nm(45～60min);进样量20μL。采用高分辨LC-MS/MS技术进行色谱峰指认。结果该方法精密度、稳定性和重复性良好。采用高分辨LC-MS/MS方法对20个共有峰中的18个色谱峰进行了定性鉴别。采用该方法测定了10批不同产地的牡丹皮,其相似度均在0.96以上。结论本实验为牡丹皮的全面质量评价奠定了基础。

Objective To establish the fingerprint of Moutan Cortex by HPLC. Methods The chromatographic fingerprint was obtained with Dikma Diamonsil C18 column （250 mm× 4.6 mm, 5 μm） and gradient eluted with acetonitrile and water （with 0.085% phosphoric acid）. The column temperature was maintained at 35 ℃, and the flow rate was 1 mL/min. The detection wavelength was 254 （0--21.5 min）, 230 （21.5--45 min）, and 254 nm （45--60 min）. The injection volume was 20 μL. The peaks were identified by LC-MS/MS. Results The method had good precision, stability, and repeatability. Eighteen chromatographic peaks among 20 common peaks were identified by LC-MS/MS. Ten batches of Moutan Cortex were determined, and the similarities were above 0.96. Conclusion The study lays the foundation for the full quality evaluation of Moutan Cortex.