新霉素发酵液提取工艺的优化研究

Studies on optimization of neomycin fermentation liquid extraction process

新霉素对热较敏感,现有提取工艺主要采用蒸发脱氨和浓缩,提取率仅达75%,残余的新霉素以废水方式排放,增加了污染物的排放。本研究通过工艺改进经732树脂动态吸附与洗脱,新霉素的交换量为142.9 mg ml-1树脂,洗脱收率高于96%,同时减少了工艺水的用量。用LSA—700树脂脱色效果优异711树脂,新霉素回收率达92.1%,色素OD值为0.38。最后通过200Da分子量的纳滤膜组件处理可有效去除物料中的NH4OH,新霉素的收率高于99.4%,副产物氨水可回收利用。优化后的新霉素提取率达88.2%,高于工厂现有水平,说明本工艺能够很好地应用于新霉素的提取。

Because neomycin is sensitive to heat, the extraction rate of the existing extraction technology by applying the evaporation deamination and concentration is only 75%. The residual neomycin is discharged as wast ewater increasing pollutant discharge. Through the improved process using dynamic adsorption and elution of 732 resin in this study, the exchange capacity of neomycin is 142.9 mg per ml resin, the elution yield is higher than 96 %, while reducing water consumption. The decoloration effect of LSA - 700 resin is better than 711 resin, whose neomycin recovery rate reaches 92.1% and pigment OD value is 0.38. Finally, the NH4OH in materials can be effe ctively removed by nanofiltration membrane of 200Da, the neomycin yield is higher than 99.4%, and the by-prod uct ammonia can be recycled. After the optimization, the neomycin extraction rate is 88.2%, higher than the existi ng level, showing this process can be well applied to neomycin extraction.