福寿螺多糖对大鼠原代免疫性肝损伤保护作用机制研究

Study of the protection of apple snail polyose against immunological liver injury induced by BCG plus LPS in rat

目的在福寿螺多糖（ASP）体内、体外抗乙肝病毒实验的基础上，进一步研究其对大鼠原代免疫性肝损伤的保护作用。方法采用卡介苗（BCG）联合脂多糖（LPS）构建大鼠原代肝细胞免疫性损伤模型。同时分别加入生理盐水、联苯双酯（DDB）及ASP（低、中、高剂量组）进行作用，收集3、6、12、24h细胞上清液，检测上清液中天冬氨酸氨基转移酶（AST）、一氧化氮（NO）含量。同法建立大鼠免疫性肝损伤模型，用ASP连续灌胃给药10天，检测血清中AST、NO。结果ASP中、高剂量组体内、体外均可不同程度地降低免疫损伤肝细胞E清液中AST、NO水平，其作用与阳性对照组药物作用相似。结论ASP对大鼠免疫性肝损伤有显著保护作用。

Objective To investigate the mechanism of the protection of apple snail polyose （ASP） against immu- nological liver injury in rat according to the study of it in vivo, in vitro anti - hepatitis B virus. Methods Immunological rat liver injury model was induced by bacillus Calmette -Guerin （BCG） plus lipopolysaccharid （LPS） in vitro and cul- tured with saline, biphenyl dimethylester （DDB） , ASP low, medium and high dose . The supernatant of cells ＂after 3, 6, 12 and 24 h incubations were taken for the detection of activities of AST and contents of NO respectively. To establish a model in vivo with the same method . Rat were treated with ASP through oral garage for 10 consecutive days and the levels of AST, NO in serum were detected. Results Apple Snail Polyose medium, high dose could decreased levels of AST and NO in different degree and different time point （ P 〈 0.05 ） in vitro and in vivo. The effection were similar with DDB. Conclusions ASP had significantly protective effects against immunological liver damage induced by BCG plus LPS in vitro and vivo.