三氧对离体肝癌细胞SMMC-7721凋亡率的影响

Effect of ozone on the apoptosis rate of liver cancer SMMC-7721 in vitro

目的探讨不同浓度医用三氧对离体肝癌细胞SMMC-7721凋亡率的影响。方法用医用三氧发生器生成3种不同浓度（20，50和80μg／ml）的三氧，分别将其通入瓶装三蒸水100ml 10min，生成不同浓度的三氧水。实验分为5组，A组为0μg／ml03，B组为20μg／mlO3，C组为50μg／mlO3，D组为80μg／mlO3，E组为对照组。将肝癌细胞SMMC-7721接种于6孔培养板，分别加入不同浓度的三氧水培养32h后消化、收集细胞到10m1的离心管中，采用AnnexinV—FITC／PI双染法染色，然后用流式细胞仪检测细胞的凋亡率。结果B、C、D组均出现SMMC-7721细胞凋亡；各组与D组比较差异均有统计学意义（P〈0．05）。结论3种不同浓度三氧化水均可导致SMMC-7721细胞凋亡，以80μg／mlO3出现的凋亡率最高。

Objective To explore the effects of ozone in different concentration on the apopto- sis rate of liver cancer SMMC-7721 in vitro. Methods Theraputic ozonation water was produced by the ozone generator and went through 100 ml bottled medical tri-distilled water for 10 min in 3 differ- ent concentration （20,50 and 80μg/ml）. There were 5 groups in this experiment. Ozone was 0 μg/ml in group A, and 20μg/ml,50 μg/ml,80 μg/ml 03 in group B,C,D respectively and E was control group. Liver cancer SMMC-7721 cells were planted in a 6 wells plates. The cells were collected into the 10 ml centrifuge tubes after 32 h with ozonation water and then dyeing with the annexin V-FITC. The apoptosis rate was detected by the flow cytometry （FCM）. Results Cells in group B,group C and group D showed apoptosis. There were significant differences between group D and other groups （P〈0.05）. Conclusion Ozonation water can induce SMMC-7721 apoptosis in 3 different concentra- tions （20, 50 and 80 μg/ml）. The highest apoptosis rate is in group D with ozone concentr- ation 80μg/ml.