摘要
目的:观察不同氧浓度环境下不同时间处理对大鼠大脑皮层细胞表达IL-1β、IL-6和TNF-α mRNA的影响。方法:原代培养新生SD大鼠大脑皮层细胞,设1%、4%两个低氧浓度和3、6 h两个低氧处理时间,处理结束后收集各组细胞,采用实时荧光定量PCR(RT-QPCR)方法检测各组细胞表达IL-1β、IL-6和TNF-αmRNA情况。结果:与相应的常氧对照组相比,1%和4%氧浓度环境处理细胞3 h和6 h时,IL-1β和TNF-αmRNA表达量均无明显变化(P>0.05);处理细胞3 h时,IL-6 mRNA表达量均无明显变化(P>0.05),但有上升的趋势,处理细胞6 h时IL-6 mRNA表达量均显著升高(P<0.05)。结论:1%和4%氧浓度环境虽均对大脑皮层细胞表达IL-1β和TNF-α mRNA无影响,但可诱导细胞表达IL-6 mRNA,且具有时间依赖性。
Objective： To investigate the effects of different time treatment on levels of IL-1β , IL-6 and TNF-α mRNA expressed by cerebral cortex cells in rat under different oxygen concentration. Methods： Primary cultured cerebral cortex cells were subjected to hypoxia （ 1% and 4% ） for 3 hours and 6 hours. The production of IL-1β , IL-6 and TNF-α mRNA were analyzed by RT-QPCR. Results： Compared with matched normoxic controls, the production of IL-1β and TNF-α mRNA had no significant difference when cells were subjected to hypoxia （1% and 4% ） for 3 hours or 6 hours; and the production of IL-6 mRNA had no significant difference when cells were subjected to hypoxia （1% and 4% ） for 3h, but had an increased trend; while the levels of IL-6 mRNA were significantly increased when subjected to hypoxia （ 1% and 4% ） for 6 hours. Conclusion： The stimulation of hypoxia （ 1% and 4% ） on the production of IL-6mRNA had a time-dependent, but hypoxia had no effect on the production of IL-1β and TNF-α mRNA.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2013年第2期141-144,共4页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(30870349)
上海市人类运动能力开发与保障重点实验室(上海体育学院)(11DZ2261100)
