运动和葡萄籽原花青素对高脂饮食大鼠血脂代谢及自由基水平的影响

The intervention effects of exercise and grape seed proanthocyanidins on lipid metabolism and free radical level in rats fed with high-fat-diet

目的:探讨长期中等负荷耐力运动和葡萄籽原花青素(GSP)干预对血脂异常及自由基水平异常的预防作用。方法:32只大鼠随机分为4组:普通饲料组(A组)、高脂饮食组(B组)、高脂饮食+GSP组(C组)、高脂饮食+运动组(D组)。B、C与D组大鼠采用高脂饮食喂养。C与D组大鼠在高脂饮食喂养的同时,分别给予GSP、中等负荷耐力跑台运动干预9周;不进行GSP干预的其他三组采用同等体重剂量安慰剂干预。比较各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)与高密度脂蛋白胆固醇(HDL-C)及超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果:B、C与D组大鼠血清TC、TG、LDL-C浓度明显升高及HDL-C浓度明显下降,血清SOD活性明显下降及MDA含量明显增加。但C、D组大鼠血清TC、TG、LDL-C浓度均比B组低,其中D组TG、LDL-C水平又比C组低;D组HDL-C浓度比B组高;C与D组大鼠血清SOD、MDA指标分别高于、低于B组。结论:长期中等负荷耐力运动和GSP干预均对高脂饮食大鼠血脂异常形成具一定抵抗作用,但GSP效果不如运动;两者均可减轻血脂异常大鼠SOD活性的下降程度,减少脂质过氧化反应。

Objective： To explore the preventive effects of long-term medium load endurance exercise and grape seed proan- thocyanidins （ GSP ） intervention on abnormal levels of lipid and free radicals. nethod：A total of 32 rats were randomly divided into 4 groups： normal diet group （ roup A）, high fat diet group （ group B）, high fat diet plus GSP group （group C）, high fat diet plus exercise group （group D）.Group B, C and D rats were fed with high-fat diet. At the same time groups C and D rats with high-fat diet feeding were respec- tively given GSP or medium load endurance run intervention for 9 weeks. In other three groups without GSP in- tervention, were intervened with the same weight dose of placebo. Rats＇ serum total cholesterol （TC）, triglyceride （TG）, low density lipoprotein cholesterol （LDL-C）, high density lipoprotein cholesterol（HDL-C） and superoxide dis- mutase（SOD）, malondialdehyde （MDA） levels were compared. Result： In groups B, C and D rats＇ serum TC, TG, LDL-C concentrations increased significantly and HDL-C concentration decreased significantly, activity of serum SOD decreased significantly and content of MDA increased obviously. In groups C and D rats＇ serum TC, TG, LDL-C concentrations were lower than those in B group,LDL-C and TG concentrations on group D were lower than those in group C. HDL-concentration of group D was higher than that in group B; in groups C and D rats, activities of serum SOD were higher than that in group B, contents of MDA were lower than that in group B. Conclusion： The intervention effect of long-term medium load endurance exercise or GSP on the informing of lip- id abnormalities in rats fed with high fat diet present a certain resistance, but the resistance effect of GSP is not so better than exerciser.Long-term medium load endurance run or GSP intervention can improve SOD activity in dyslipidemia rat, thus reducing the lipid peroxidation. Author＇s address Department of Physical Education,Guangxi Normal University,Guilin, 541004