Genipin交联丝素蛋白纳米纤维膜的制备与性能

Preparation and properties of Genipin cross-linked silk fibroin nanofibrous membranes

利用Genipin对再生丝素蛋白进行交联改性,并通过静电纺丝法制备交联的丝素蛋白纳米纤维膜。利用场发射扫描电镜、红外光谱仪、X射线衍射仪、热重分析仪以及拉力机等对其结构与性能进行表征与测试。结果表明,随着交联剂Genipin质量比的增加,交联度增加,静电纺丝素蛋白纳米纤维平均直径增大,标准偏差增大;Genipin交联对丝素蛋白纳米纤维结晶结构影响不大,但热性能提高;常温条件下,随着Genipin质量比从2%提高至15%,丝素蛋白纳米纤维膜的力学性能逐渐增强,质量比为10%时,其力学性能较好,拉伸强度和断裂应变分别为19.6MPa和5.9%;随着试验温度从40℃升高到200℃,丝素蛋白纳米纤维膜的拉伸强度和断裂应变先增大然后减小,当试验温度为80℃时,其力学性能较好,拉伸强度和断裂应变分别为41.6MPa和8.6%。

The regenerated silk fibroin（SF） was modified by Genipin cross-linking,and then was made into cross-linked nanofibrous membranes via electrospinning.The structure and properties of nanofibers were characterized and tested by use of FE-SEM,FTIR,XRD,TGA and tensile tester.The results show that the degree of cross-linking,average diameter of nanofibers and standard deviation increase with the increasing of Genipin mass ratio.And the crystal structure of SF nanofibers is not influenced by Genipin cross-linking,but thermal properties are improved.Under room temperature,the mechanical properties of SF nanofibrous membranes are enhanced with the increasing of Genipin mass ratio from 2% to 15%.And the mechanical property is better when the Genipin mass ratio is 10%,the tensile strength and breaking strain are 19.6 MPa and 5.9% respectively.The tensile strength and breaking strain of SF nanofibrous membranes first increase and then decrease with the rising of tensile temperature from 40 ℃ to 200 ℃,the mechanical property is better at 80 ℃,the tensile strength and breaking strain are 41.6 MPa and 8.6% respectively.