手针刺激“足三里”穴局部肥大细胞活动与外周神经放电的相关性研究

Relationship between Regional Mast Cell Activity and Peripheral Nerve Discharges during Ma-nual Acupuncture Stimulation of "Zusanli"(ST 36)

目的:探讨穴位处肥大细胞的激活及穴区胶原与支配该区域的神经束放电变化之间的关系。方法:雄性SD大鼠30只,随机分为空白组、对照组、手针足三里组、手针+色甘酸钠组(穴区肥大细胞拮抗)、手针+胶原酶组(穴区胶原纤维溶解),每组6只。动物麻醉后,分离左侧"足三里"穴区坐骨神经干,手针提插刺激大鼠"足三里"穴20min,记录刺激前、刺激时和刺激后神经束诱发放电信号。对穴位组织进行组织学观察,统计肥大细胞脱颗粒率。结果:与对照组、手针+色甘酸钠组和手针+胶原酶组相比,手针足三里组诱发的坐骨神经放电活动明显增强(P<0.01);同时手针足三里组肥大细胞脱颗粒率显著升高(P<0.01),而手针+色甘酸钠组和手针+胶原酶组肥大细胞脱颗粒率与对照组相比差异无统计学意义(P>0.05)。结论:手针刺激大鼠"足三里"穴位可诱发支配该区域的外周神经束放电;穴位区注射色甘酸钠可抑制肥大细胞脱颗粒,针刺引起的外周神经放电减弱;穴位区注射胶原酶阻断针刺应力在穴位组织中传输,可以抑制肥大细胞脱颗粒,同样,针刺引起的外周神经放电也减弱。

Objective To observe changes of discharges of the sciatic nerve branch and mast cell activities and colla- gen fibers in the acupoint area during manual acupuncture stimulation of ＂Zusanli＂（ST 36）, so as to reveal the relationship be- tween peripheral nerve and mast cell activities. Methods A total of 30 male SD rats were divided into normal, acupuncture control Can acupuncture needle was inserted into ST 36 without manipulation）, manual acupuncture （MA）, disodium cromoglycate （DSCG, suppressing mast cell activity） plus acupuncture （MA＋ DSCG） and collagenase （dissolving the collagen fibers） plus acu- puncture （MA＋ collagenase） groups （6 rats/group）. After dissection of a branch of the sciatic nerve innervating ST 36 region in the left hind-limb under anesthesia, the ipsilateral ST 36 was stimulated by manipulating the acupuncture needle for 20 min. Dis- charges of the sciatic nerve branch were recorded by using a pair of metal electrodes and data acquisition system （Power Lab）. Skin and muscle tissues of ST 36 area were sampled, sliced and stained with Toluidine Blue for detecting the number of degranu- lated mast cells. Results Compared with the control group, the mean power spectrum of discharges of the sciatic nerve and the mean rates of the degranulated mast cells in ＂Zusanli＂ （ST 36） area in the MA group were significantly increased （P^0.01）. Whereas the mean power spectrum of discharges of the sciatic nerve and the mean degranulation rates of mast cells were consi- derably lower in the MA ＋ DSCG group and MA＋ collagenase group than in the MA group （P^0.01）. No significant differences were found between the normal and control groups, and between the MA-t-DSCG and MA-t-collagenase groups in the mean power density and degranulation rates of mast cells （P〉0.05）. Conclusion Manual acupuncture stimulation of ST 36 can significantly potentiate the discharge activity of the sciatic nerve and induce degranulation of mast cells at the same time, suggesting an in- volvement of mast cells in initiating acupuncture signals by peripheral sensory nerve.