艾灸对类风湿性关节炎家兔滑膜细胞JAK-STAT通路负反馈调节家族细胞因子信号抑制因子的影响

Effects of Moxibustion Intervention on Inflammatory Reactions and Expression of Suppressor of Cytokine Signaling Proteins of Synovium Cells in Rheumatoid Arthritis Rabbits

目的:观察艾灸对实验性类风湿性关节炎(RA)家兔的抗炎消肿作用,探索艾灸抗炎效应的滑膜细胞JAK-STAT信号通路负反馈调节机制。方法:日本大耳白兔随机分为对照组、模型组和艾灸组,各14只。福氏完全佐剂注入兔双后膝关节腔复制RA模型。艾灸组动物采用艾粒施灸两侧"肾俞"穴各5壮,每日1次,6d为一疗程,共治疗3个疗程。治疗后观测各动物左、右后膝关节周长,运用免疫组化法检测细胞因子信号抑制因子1(SOCS 1)、SOCS 3的表达。结果:造模后各个时间点模型组动物的左、右后膝关节周长均较对照组明显增加(P<0.01),治疗后艾灸组动物左、右后膝关节周长均较模型组降低(P<0.05)。模型组与对照组比较,滑膜细胞SOCS 1、SOCS 3蛋白含量显著升高(P<0.01);艾灸组与模型组比较,滑膜细胞SOCS 1、SOCS 3蛋白含量降低(P<0.01)。结论:艾灸对实验性RA具有抗炎效应,艾灸对滑膜细胞JAK-STAT信号通路中相关负反馈调节因子SOCS 1、SOCS 3表达的调节可能是其实现抗炎效应的机制之一。

Objective To observe the effect of moxibustion intervention on inflammatory reactions and expression of suppressor of cyfokine signaling 1 （SOCS 1） and SOCS 2 [-Which are involved in inhibition of the Janus Kinase-signal transducer and activator of transcrip-tion （JAK/STAT signaling pathway and in sffenuation of cytokine signaling）] in synovium cells of the hind-knee joint in rheumatoid arthritis （RA） rabbits, so as to study its mechanism underlying improvement of RA. Methods Forty-two Japanese big-ear white rabbits were randomized into control, model and moxibustion groups respectively, with 14 cases in each group. RA model was established by injection of Freund＇s Complete Adjuvant （0.5 mL/kg） into the rabbits＇ bilateral hind- knee joint cavities. Moxibustion was applied to bilateral ＂Shenshu＂ （BL 23） areas, 5 cones every time, once daily for 3 weeks except the Sundays. The perimeters of rabbits＇ hind legs were measured before and after modeling and after the therapy. The synovial tissue of joint was sampled for analyzing the expression levels of SOCS 1 and SOCS 3 by immunohistochemistry. Results Before the therapy, the perimeters of bilateral knee joints of the control, model and moxibustion groups were of no statistical sig- nificance （P〉0.05）. In comparison with the control group, the perimeters of bilateral knee joints were significantly increased on day 1, 7, 14 and 21 in the model group （P〈0.01）. Compared with the model group, the perimeters of bilateral knee joints in the moxibustion group were significantly decreased （P〈0.05）, suggesting an improvement of the inflammatory reaction after moxi- bustion intervention. Correspondingly, synovial sees 1 and sees 3 expression levels were remarkabeiy higher in the model group than in the control group （P〈0.01）, and obviously decreased in the moxibustion group compared with the model group （P〈0.01）. Conclusions Moxibustien intervention has an anti-inflammatory and detumescent effects in RA rabbits, which may be closely associated with its effects in down-regulating expression of sees 1 and sees 3 proteins by suppressing negative feed- back regulatory JAK/STAT pathway in synovial cells.