糖尿病视网膜表面膜细胞成分的研被引量：2

Investigation of cellular constituent in diabetic epiretinal membranes

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摘要目的探讨在视网膜内界膜完整的情况下糖尿病视网膜表面膜中的主要细胞成分,推测这些增殖细胞的来源,为糖尿病视网膜表面膜形成的病理机制提供新的思路。方法视网膜表面膜为糖尿病视网膜表面膜,10眼来自玻璃体视网膜手术眼,2眼取自尸体眼(生前为糖尿病视网膜表面膜眼),这些病例中内界膜都保持完整。通过HE染色、免疫组织化学和电镜检查研究表面膜的细胞成分。结果神经纤维酸性蛋白、S-100蛋白、神经元特异性烯醇化酶、波形蛋白在视网膜表面膜表达阳性,提示表面膜中的主要细胞成分为星形胶质细胞。电镜观察可见具有规则圆核、细胞质中有成熟线粒体和高尔基器的星形胶质细胞,细胞表面见长的胞突和微绒毛,表面膜中见纤维性胶原。结论星形胶质细胞可能在糖尿病视网膜表面膜形成中发挥重要的作用。内界膜完整的情况下出现的表面膜,星形胶质细胞为其主要细胞成分。 Objective To investigate the main cellular constituent in diabetic epiretinal membranes with intact internal limiting membranes and postulate reasons for proliferation of those cells,and to provide new idea for exploring the pathological mechanism of the shaping of diabetic epiretinal membrane.Methods A total of 12 eyes with intact diabetic epiretinal membranes were selected,among which 10 eyes were collected from retinal-vitreous surgeries,2 eyes were collected from postmortem patients.Then the cellular constituent in the diabetic epiretinal membranes were determined by HE staining,immunohistochemical staining,and electron microscopy.Results Tests results of glial fibrillary acid protein（GFAP）,S-100 protein,neural specific enolase（NSE）and vimentin were positive,which suggested the main cellular constituent in the diabetic epiretinal membranes were astrocytes.Astrocytes with regular rounded nuclei and mature mitochondria and Golgi apparatus in the cytoplasm were observed by electron microscopy.Fibrillar collagen was observed in the diabetic epiretinal membranes.Conclusion Astrocytes paly an important role in the shaping of diabetic epiretinal membranes.Astrocytes are the main cellular constituent in diabetic epiretinal membranes with intact internal limiting membranes.

作者辛晓蓉巩天祥

机构地区青海省红十字医院眼科成都血液中心研究室

出处《眼科新进展》 CAS 北大核心 2013年第4期309-311,315,共4页 Recent Advances in Ophthalmology

基金国家自然科学基金项目(编号:81160122)~~

关键词糖尿病视网膜表面膜内界膜星形胶质细胞神经纤维酸性蛋白 S-100蛋白神经元特异性烯醇化酶波形蛋白 diabetic epiretinal membrane internal limiting membrane astrocytes glial fibrillary acid protein S-100 protein neural specific enolase vimentin

分类号 R774.1 [医药卫生—眼科]

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