红花穴位离子导入治疗外伤性玻璃体积血实验研究

Treatment of honghua injection acupoint iontophoresis on traumatic vitreous hemorrhage

目的探讨中药红花注射液眼部穴位离子导入的药代动力学作用以及对实验性外伤性玻璃体积血的治疗作用。方法将兔实验性外伤性玻璃体积血模型18只（36只眼）随机分成2组，治疗组予红花注射液经睛明、承泣、攒竹、劳宫穴位离子导入；对照组不做任何治疗，观察玻璃体积血的自然转归。分不同时间点抽取兔房水和玻璃体，用高效液相色谱法测定房水和玻璃体中的药物质量浓度。用检眼镜观察眼底玻璃体积血吸收情况，并行眼底照相。结果兔眼房水线性回归方程为Y=32．486X-5．154，（r=0．9989，P〈0．01）；玻璃体线性回归方程为Y=30．462X-5．024，（，=0．9951，P〈0．01）；兔房水和玻璃体内羟基红花黄色素A的质量浓度在0．5h和2h差异均无统计学意义（P〉0．05），5h时差异有统计学意义（P〈0．01）；治疗后治疗组眼底可见度明显优于对照组（P〈0．01）。结论红花穴位离子导人可促进兔眼外伤性玻璃体积血的吸收，缩短病程，是一种简易有效的眼局部治疗方法。

Objective To investigate the effect and pharmcokinetic role of honghua injection acu- point iontophoresis on traumatic vitreous hemorrhage. Methods The traumatic vitreous hemorrhage model was induced by injecting into vitreous of 0.2 mL autohemorrhage in 36 eyes of 18 New Zea- land white rabbits. 2 ml of honghua solution was iontophorezed via acupoint iontophoresis in the Qingming, Chengqi, Cuanzhu, Laogong for 15 minutes per time and twice per day for 10 times in honghua-treated group （9 rabbits）, and 9 model rabbits were as control group. Aqueous humor and vitreous were withdrawn at different time, and the drug concentration in aqueous humor and vitreous were detected with high-pressure liquid chromatography （HPLC）. The absorption status of vitreous hemorrhage was clinically evaluated by ophthalmoscope. Results The aqueous humor regression equation of honghua peak area （Y） and honghua concentration （X） was Y=32.486 X-5.154, present- ing a good linear correlation between them （r =0.9989, P 〈0.01）. The vitreous regression equation of honghua peak area （Y） and honghua concentration （X） was Y=30.462 X-5.024, presenting a good linear correlation between them （r =0.9951, P 〈0.01）. There was no significant difference in honghua concentration between vitreous and aqueous humor in 0.5 hours and 2 hours （P 〉0.05）, but signifi- cant difference was found in 5 hours （P 〈0.01）. No obvious difference was seerl in visuality of fun- dus before treatment between the two groups. In honghua-treated group, the absorption of vitreous hemorrhage was quicker than in the control group, and the number of visual fundus was remarkably increased in comparison with control group, showing that the effect of honghua injection-iontophoresis was better than that in control group. Conclusions For traumatic vitreous hemorrhage, honghua injection-iontophoresis is an effective and simple approach to accelerating the absorption of vitreous hemorrhage and shorten the course of treatment.