大豆黄素对有机氯类农药诱导乳腺癌细胞增殖效应的抑制作用

Inhibitory action of the daidzeinon organochlorine pesticide-induced MCF-7 breast cancercell multiplication

目的:探讨大豆黄素对有机氯农药诱导乳腺癌MCF-7细胞增殖效应的抑制作用。方法:取对数生长期的MCF-7细胞,分别设含不同浓度的大豆黄素、氯丹、β-六六六(β-HCH)、邻,对’-滴滴涕(o,p'-DDT)单独染毒组以及100μmol/L大豆黄素与上述各农药联合作用组,并设溶剂对照(0.1%二甲基亚砜)组。培养48h后,采用噻唑蓝(MTT)实验检测MCF-7细胞的增殖情况。结果:与溶剂对照组相比,100μmol/L的大豆黄素单独染毒组使MCF-7细胞的增殖率下降,不同浓度的有机氯农药单独染毒使MCF-7细胞的增殖率升高,差异均有统计学意义(P<0.05)。与相应不同浓度的有机氯农药单独组相比,联合染毒组MCF-7细胞的增殖率明显下降,差异均有统计学意义(P<0.05)。结论:大豆黄素能够抑制不同浓度有机氯农药诱导的MCF-7细胞的增殖效应。

OBJECTIVE： To investigate the combined action of inhibitory daidzein and organochlorine pesticideson MCF-7 cell multiplication. METHODS：MCF-7 human breast carcinoma cells in logarithmic growth phase were exposed to each of daidzein,chlordane,β-HCH,o,p＇-DDT in different concentration alone and another group with 100 μmol/L daidzein combined with each pesticide. A solvent control group （0.1% DMSO） was set up.After 48 h of incubation,the cell multiplication was assessed by MTT. RESULTS：Compared with solvent group,the 100 μmol/L daidzein only group reduced cell multiplication. All different concentrations of each organochlorine pesticide could increase cell multiplication （P0.05）. Compared with the single agent treatment group,the rate of cell multiplication in combined treatment group was significantly reduced （P0.05）. CONCLUSION：Daidzein could inhibit cell multiplication induced by different concentrations of organochlorine pesticides.