摘要
将靶向猪繁殖与呼吸综合征病毒(PRRSV)的shRNA表达盒插入pEGFP-N1的EcoO109I酶切位点构建重组转基因载体pEGFP-G1,并在转基因Marc-145细胞验证其抑制PRRSV复制的功能。用pEGFP-G1转染Marc-145细胞24 h后感染欧洲型PRRSV,病毒感染后每天观察细胞病变(CPE)情况。试验结果表明:所获pEGFP-G1可明显抑制PRRSV复制。
The PRRSV-targeting shRNA expression box was inserted into the EcoOl09I site of pEGFP-N1 to construct the recombinant transgenic vector pEGFP-G1 and its function of inhibiting PRRSV replication was verified in the transgenic Marc-145 cells. The Marc-145 cells were infected with Europe-type PRRSV at 24 h after transfected with pEGFP-NI,and the cytopathic effect was ob- served daily. The result showed that as compared with the control the pEGFP-G1 in the MARC-145 cells could significantly inhibit PRRSV replication.
出处
《上海农业学报》
CSCD
北大核心
2013年第2期9-13,共5页
Acta Agriculturae Shanghai
基金
博士学科点专项科研基金(20100097110008)
江苏省研究生科研创新计划项目(CXZZ11-0682)
江苏高校优势学科建设工程项目资助
