SOX2在结直肠癌组织中的表达与临床病理学意义

Expression and Clinical Pathological Significance of SOX2 in Colorectal Carcinoma

目的:探讨结直肠癌组织中SOX2的表达及与结直肠癌临床病理特征的关系,为指导结直肠癌的临床治疗及预后判断提供重要依据。方法:收集150例结直肠癌组织标本,采用LSAB免疫组化法检测癌组织中SOX2的表达。采用实时RT-PCR检测其中3例癌组织及癌旁组织中SOX2mRNA的表达情况,分析SOX2蛋白表达与肿瘤临床病理的相关性。结果:SOX2阳性表达主要位于结直肠癌癌细胞的细胞核内,且在127例呈中高表达(表达率84.6%)显著高于例癌旁结直肠组织中的高表达(8%,P<0.01)。SOX2mRNA在结直肠癌组织的表达水平是癌旁结直肠组织的2.5～15倍。SOX2蛋白表达与结直肠癌患者的年龄、性别以及肿瘤部位、大小等无相关性(P>0.05),但与结直肠癌的浸润深度、淋巴结转移、Dukes’分期有显著相关性(P<0.01)。结论:结直肠癌组织中SOX2蛋白和mRNA的表达显著高于癌旁结直肠组织,其表达与结直肠癌的浸润深度、分化程度、淋巴结转移、Dukes’分期有关。

Objective To observe the expression of SOX2 in colorectal carcinoma and explore its clinico- pathological significance. Methods Samples were collected from 150 patients with colorectal carcinoma. Im- munohistochemistry of LASB was used to detect the expression of SOX2, and real time RT-PCR was used to de- tect the expression of SOX2 in the remaining 3 samples to analyze the relationship of expression of SOX2 with colorectal carcinomas. Results The SOX2 was found to be expressed in nuclear oi colorectal carcinomas and carcinoma side mucosal epithelium. of SOX2 was detected in a significan portion of cancer tissue （84.6%） than The expression tly greater pro- that of carcino-ma side tissue （8%） . SOX2 mRNA levels in colorectal carcinoma tissue was 2.5 to 15 fold higher than that in carcinoma side tissue. By statistical analysis , the expression levels of SOX2 in colorectal carcinoma were not correlated with clinical pathological characteristics , such as age, gender and volume（P 〉 0.05） . but correlated with the infiltration depth, metastasis, and Dukes＇stages （P 〈 0.01）. Conclusion The expression of SOX2 is stronger in colorectal carcinoma than in carcinoma side tissue. The expression of SOX2 in colorectal carcinoma is correlated with some characteristics of clinical pathology.